HBsAg-(6His) Viral Like Particles

$650.00

SKU: VLP004 Category:

Description

HBsAg  is a surface antigen of the hepatitis B virus (see HBV scheme below). HBsAg can be found in blood which is a sign of infection. If it is found, along with its specific antibody, It means the person is infected by HBV and is infectious.

HBV structure scheme

HBsAg-(6His) (click to see its protein sequence) Viral Like Particles was packaged in HEK293T cells using our proprietary technology. It is the replication-incompetent particles in which the HBsAg (Surface antigen) of Hepatitis B virus (with 6His-tag at its C-terminal) was pseudo-typed as the lentiviral particles’ surface envelope protein.  [Note: This VLP has the HBsAg antigen expressed on the particle’s surface.  If you want to over-express the HBsAg antigen in your desired cell types, please use lentivirus product, CAT#: LVP1402-PBS.]

This VLP packaged a non-sense sequence (Null) as the particle’s virion genomic material, to maintain the intact of the particles.

Applications:

HBsAg VLP was assembled as surface antigens which can be used as the ready to use HBsAg antigen, or for  raising the specific antibodies, for the validation of anti-HBsAg antibodies, in vaccine development and other validation assays. This VLP can also be detected by the anti-C-terminal 6His-tag antibody.

The VLP’s titer was measured via ELISA P24 assay. Every single particle assembled many HBsAg molecules at its surface. See more details in Product Manual.

Amount: 200ul/vial at 1×108 VP/ml.

CAT#: VLP004