This HEK293/ Cas9 expression Stable Cell Line is transformed from the HEK293 cell line, stably expressing the nuclear penetrating humanized wild-type Cas9 endonuclease (Streptococcus pyogenes). The Cas9 enzyme is driven by our engineered super strong CMV promoter (suCMV). It contains the Puromycin antibiotic selection under the RSV promoter. see the expression cassette below that integrated into cell line’s genome. Product Manual.
This CRISPR-ready (Cas9 expression) stable cell lines make genomic editing easier than ever. You simply deliver the target-specific gRNA into the CRISPR-ready cell line and select the knock-out or knock-in cells for your desired target. No need to do cas9 containing CRISPR cloning, and no worry about the hard-to-delivered Cas9 constructs. You only need to generate the much easier in cloning, and much smaller in size of the target-gRNA constructs. GenTarget also provides services to generate your target-specific, ready-to-use gRNA lentivirus. Please contact us if you need the CRISPR gRNA lentivirus services.
Sold at: 1 vial x (2 x 106 cells)/vial ,