Description
Color–Switch (Red to Green), CRE reporter stable cell line:
This cell line was transformed from HEK293 Cell line, expressing “LoxP-mCherry-stop-LoxP-GFP” cassette under CMV promoter (see Color-Switch cassette below). Cell line has the Blasticidin antibiotic resistance gene under RSV promoter (not shown in the scheme).
Applications:
- This CRE reporting cell lines is used to monitor the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the recombination event by your CRE enzyme (your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE protein) In vivo and In vitro .
- It is a convenient tool to verify your CRE-loxP based system.
- It can also used to verify your CRISPR editing efficiency by targeting at LoxPs and mCherry/GFP region (please be advised, we do not provide any sequence information unless a license is purchased for this cell line).
How it works?
The cells initially express strong mCherry fluorescence. The downstream GFP is not expressed due to the presence of a stop codon upstream. Upon delivery of Cre recombinase into the nucleus, the DNA fragment between the two LoxP sites—including the mCherry cassette and stop codon—is excised. This activates GFP expression, resulting in a visible switch from Red to Green fluorescence. The percentage of GFP-positive cells reflects the efficiency of Cre recombination and can be easily quantified using fluorescence microscopy or flow cytometry (FACS).
Sample images of CRE-loxP recombination detection:
Left panel / without CRE: CRE reporter cell line (Cat#: SC103-Puro) was cultured in 24-well plate. Right panel / with CRE: 50ul of CRE expression lentivirus (Cat#: LVP339-PBS) was added into the cells in one well in 24-well/plate. Images were taken at ~ 48 hours after the addition of CRE expression lentivirus.
Please click here for Product Manual.
Sold at: 1 vial x (2 x 106 cells)/vial
Cat#: SC103-Bsd
