Transformed from HEK293 cells express tetracycline repressor (TetR) gene. TetR was expressed under suCMV promoter. A GFP-Blasticidin fusion dual marker was expressed under RSV promoter (all cells demonstrate high GFP fluorescent signal). The following expression cassette was inserted into cell line’s genome.
The TetR stable cell lines can be used together with Gentarget’s cDNA expression lentivirus for the inducible target expression, or for generation of the target specific inducible expression cell lines. See How inducible expression works.
Sold at: 1 vial x (>2 x 106 cells)/vial, see Product Manual for details.