Human HCT116 / GFP Fluorescent Stable Cells

$2,000.00

Human HCT116 cell line expresses enhanced GFP fluorescent report, containing puromycin selection.

SKU: SC088-G Categories: ,

Description

Human HCT116 cell line is derived from human colorectal carcinoma (Adult male). It has a mutation in codon 13 of the ras proto-oncogene. The cells are adherent with an epithelial morphology. HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression.

HCT116 cell line was used in tumorigenicity studies in vitro and in vivo. It forms primary tumor after implantation into nude mice, which makes it a great tool for studying the mechanisms of tumor growth and metastasis.

This GFP / human HCT116 cell line expresses the codon-optimized monomer GFP fluorescent marker. RFP was expressed under the enhanced EF1a Promoter. Cell line carries the Puromycin resistance.

The following expression cassette was integrated into the cell line’s genome:

Vector scheme express GFP under EF1a promoter

Each cell showed RFP fluorescent signal. see a sample image below:

HCT116 cell line express GFP reporter

Sold at:  1 vial x (2 x 106 cells)/vial, see details in Product Manual.

Cat# SC088-G