This human B lymphocyte host cell line (RPMI 8226) derived from 61 years male, with surface antigen profile as: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +. It has the Luciferase and GFP constitutively co-expressed under EF1a promoter as two separate protein (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome.
Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal via Luc assay (Note: It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells).
Sold at: 1 vial x (2 x 106 cells)/vial, see details in Product Manual.