The Human T47D cell line was derived from the pleural effusion of a ductal carcinoma of the breast of an 54-year-old female human patient. This T47D cell line has been used in the studies of the effects of progesterone on breast cancer and the corresponding transcriptional regulation caused by introduced drugs, in the anti-proliferative effects of potential drugs, in migration and podosome formation. T47D has proven useful in breast cancer studies and bone metastasis.
Gentarget generated the Luciferase /GFP dual reporter cell line, transformed from human T47D cell line by lentivirus transduction. Its Luciferase and GFP was constitutively co-expressed under EF1a promoter as two separate protein (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome.
Each cell demonstrates strong GFP fluorescent signal (see sample image below) and luciferase assay signal via Luc assay
(Note: It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells).
Sold at: 1 vial x (2 x 106 cells)/vial, see details in Product Manual.