Pre-made RFP lentiviral particles containing codon optimized Red Fluorescent Protein (RFP) marker under suCMV promoter. A Neomycin (G418) antibiotic selection under Rsv promoter allows to select transduced fluorescent positive cells.
See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create RFP expression stable cell line (via Neomycin selection);
Amount: 1 x 107 IFU/ml x 200ul in in DMEM medium with 10% FBS and 60ug/ml of polybrene.