Pre-made shRNA knockdown lentivirus for human P53 gene (NM_000546). It contains a GFP-Puromycin dual selection marker. GFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Puromycin.
shRNA sequence was designed in human P53 codon (ORF) region, demonstrating greater than 75% knockdown levels for human P53 expression. This lentivirus is provided in DMEM medium with 10% FBS and 10x Polybrene, as 200ul /each.
Note: Its matched negative control shRNA lentivirus is: CAT# H1(shRNA-Ctr)-GP.
Please see Product Manual for more details.
Amount: 200ul/tube at (1 x 10 7 IFU/ml) in DMEM medium