Premade shRNA lentivirus, knockdown human P53 gene (NM_000546). It contains a RFP-Puromycin dual selection marker. RFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Puromycin.
shRNA sequence was designed in human P53 codon (ORF) region, demonstrating greater than 75% knockdown levels for endogenous human P53 expression. This is the concentrated shRNA lentiviurs, provided in PBS solution as 200ul/vial at 5 x10e7 IFU/ml, used for hard-to-transduced cell types or for serum-free cell culture.
Note: Its matched negative control shRNA lentivirus is: CAT# H1(shRNA-Ctr)-RP-PBS.
Please see Product Manual for more details.
Amount: 200ul/tube at (5 x 10 7 IFU/ml) in PBS