Premade optional inducible shRNA lentivirus, knockdown firefly luciferase gene. Particles contains a GFP-Puromycin dual selection marker. GFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Puromycin.
shRNA sequence was designed in firefly luciferase codon region, demonstrating greater than 75% knockdown level for exogenous (trasnfected luciferase expression).This is the concentrated shRNA lentiviurs, provided in PBS solution as 200ul/vial at 5 x10e7 IFU/ml, used for hard-to-transduced cell types or for serum-free cell culture.
Note: Its matched negative control shRNA lentivirus is: CAT# H1(shRNA-Ctr)-GP-PBS.
Please see product manual for more details.
Amount: 200ul/tube at (1 x 10 7 IFU/ml) in PBS