Premade optional inducible negative control shRNA expression particles under human H1 promoter. Negative shRNA controls containing a insert that designed has no homogous to any human or mouse transcripts (should not target any known human or mouse genes). These controls serve as a useful reference for interpretation of knockdown results. It serves as the negative controls for target knockdown validation.
Particles contains a GFP-Blasticidin dual selection marker. GFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Blasticidin.
Please see product manual for more details.
Amount: 200ul/tube at (1 x 10 7 IFU/ml) in DMEM medium with 10% FBS with 10x polybrene (60 ug/ml).