Pre-made optional inducible shRNA lentivirus, knockdown firefly luciferase gene. Particles contains a RFP-Blasticidin dual selection marker. RFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Blasticidin.
shRNA sequence was designed in firefly luciferase gene codon region, demonstrating greater than 75% knockdown levels for exogenous (trasnfected luciferase expression). This is the concentrated shRNA lentiviurs, provided in PBS solution as 200ul/vial at 5 x10e7 IFU/ml, used for hard-to-transduced cell types or for serum-free cell culture.
Note: Its matched negative control shRNA lentivirus is: CAT# H1(shRNA-Ctr)-RB-PBS
Please see product manual for more details.
Amount: 200ul/tube at (1 x 10 7 IFU/ml) in PBS