Pre-made lentiviral particles expressing SV40 large T-antigen gene under an enhanced EF1a promoter. A RFP-Puromycin fusion dual marker was expressed under RSV promoter, which allows to select, by blasticidin antibiotic or sort via GFP signal, the transduced cells for long term expression (Dual markers). RFP signal provide a convenient transduction monitoring for virus perfomrance.
SV40 large T-antigen causes cell immortalization and allow to produce high-titer lentivirus in your cell line. It was natively expressed without any tags. See product manual (.pdf).
Amount: This is the concentrated lentivirus, provided in PBS solution as 200ul/per vial at 1 x 108 IFU/ml