TCR-(CD1d-α-GalCer) (Clone NKT15) (GFP-Puro)

$790.00

Lentivirus express TCR recognizing α-GalCer (Clone NTK15), containing GFPPuromycin dual selection. 

SKU: LVP1688 Category:

Description

Lentivirus express TCR (a-GalCer) (Clone NTK15), containing no any antibiotic selection. This TCR actives the T-Cells with the CD1D lipid Antigen-activated antibody recognizing galactosylceramide (GalCer) (Clone (NKT15). It is often used to engineer invariant natural killer T (iNKT) cells

The TCR construct express the antibody specifically recognizes the lipid-based antigen, α-galactosylceramide (α-GalCer) (presented by CD1d). The TCR α chain variable region with a transmembrane (TM) domain  and β chain variable region with a transmembrane (TM) domain, are linked by F2A element, as “TCRα(V)/Tm-F2A-TCRβ(V)/Tm

The “target-specific TCRαV and TCRβV sequences” are derived from the verified clones according to published literatures. It also transduces a GFPPuromycin (fluorescent-antibiotic fusion ) dual selection gene under the separate RSV promoter, which allow to enrich or select the transduced cells via GFP sorting or Puromycin killing. see the Lentivector scheme below..

TCR Lentivector map (Gentarget Inc)

About α-Galactosylceramide (α-GalCer):

α-GalCer is a glycolipid antigen that can bind to CD1d molecules. α-GalCer is a potent activator of invariant NKT (iNKT) cells. When α-GalCer is presented by CD1d, it cells activates NKT cells, a unique subset of T cells that bridge the innate and adaptive immune responses. α-GalCer has been utilized extensively due to its ability to induce potent activation of mouse and human iNKT cells. References: Ref 1, REF 2, Ref 3

see details in Product Manual.

Amount: 200 ul/vial at titer of 1×108 IFU/ml, concentrated lentivirus provided in PBS solution, premixed with 10x polybrene (60ug/ml), premade, ready to ship in dry-ice package.

CAT#: LVP1688