Yellow fever virus (YFV) is an enveloped virus. Its Envelope protein (E protein) is involved in many events, such as receptor binding site for viral attachment, fusion, penetration, hemagglutination, host range and cell tropism. It also has an important role in immunological anti-virus response, eliciting neutralizing antibodies and inducing protective response.
YFV Envelope (click to see its protein sequence) Viral Like Particles was packaged in HEK293T cells using our proprietary technology. It is the replication-incompetent particles in which the YFV Envelope (with 6His-tag at its C-terminal) was pseudo-typed as the lentiviral particles’ surface envelope protein. [Note: This VLP has the YFV Envelope antigen expressed on the particle’s surface. If you want to over-express the YFV Envelope antigen in your desired cell types, please use lentivirus product, CAT#: LVP1407-PBS.]
This VLP packaged a non-sense sequence (Null) as the particle’s virion genomic material, to maintain the intact of the particles.
YFV Envelope was assembled as surface antigens which can be used as the ready to use YFV Envelope antigen, or for raising the specific antibodies, for the validation of anti-YFV Envelope antibodies, in vaccine development and other validation assays. This VLP can also be detected by the anti-C-terminal 6His-tag antibody.
The VLP’s titer was measured via ELISA P24 assay. Every single particle assembled many envelope molecules at its surface. See more details in Product Manual.
Amount: 200ul/vial at 1×108 VP/ml.