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Home / microRNA lentivirus

microRNA lentivirus

Pre-Made Human and Mouse miRNA expression Lentivirus

Human or mouse microRNA precursors with its native context sequences (upstream and downstream flanking genomic sequences) were PCR amplified and cloned into a pLenti-TetCMV(GFP-Stop-3UTR/miRNA)-Rsv (Puro) lentivector. The GFP and pre-miRNA are co-transcribed under the same promoter, the optional inducible CMV promoter. GFP provides a convenient indicator for miRNA expression levels, and a Puromycin antibiotic selection marker (under a Rsv promoter) provides the selection method for long term stable expression. See the scheme below for the core vector structure.

miRNA-exp-map

A sequence-verified miRNA expression lentivector was co-transfected with lentiviral packaging plasmids (Cat#: HT-pack) into a lentivirus production cell line (Cat#: TLV-C). The VSV-G pseudotype lentivirus was packaged in DMEM medium with 10% serum according to GenTarget’s virus production protocols, and then the virus was filtered through a 0.45 µm filter. See product manual for details. 

Each order contains: 

  1. 0.5 ml of pre-made specific miRNA lentivirus
  2. 0.5 ml of negative control miRNA lentivirus
  3. miRNA expression product manual 

Virus titer: 1×107 IFU/ml 

Shipping: overnight shipment on dry-ice.

Click to see: Human miRNA expression lentivirus product list.

Click to see: Mouse miRNA expression lentivirus product list.

Note: If you do not find the miRNA you need here, You can place a custom order for lentivirus expressing any miRNAs that are listed in the miRBase database. Service quote may be requested by Email or Fax.

NameSKUPriceBuy
Custom-Human miRNA expression lentivirusH-GFP-miR$800.00
Custom-Mouse miRNA expression lentivirusM-GFP-miR$800.00
hsa-let-7f-5p (MIMAT0000067) miRNA expression lentivirusmiR#11$800.00
hsa-miR-101-3p (MIMAT0000099) miRNA expression lentivirusmiR#73$800.00
hsa-miR-125b-5p (MIMAT0000423) miRNA expression lentivirusmiR#178$800.00
hsa-miR-155-5p (MIMAT0000646) miRNA expression lentivirusmiR#246$800.00
hsa-miR-193a-3p (MIMAT0000495) miRNA expression lentivirusmiR#238$800.00
hsa-miR-34a-3p (MIMAT0004557) miRNA expression lentivirusmiR#113$800.00
hsa-miR-34a-5p (MIMAT0000255) miRNA expression lentivirusmiR#112$800.00
hsa-miR-375 (MI0000783) miRNA expression lentivirusmiR#306$800.00
hsa-miR-4284 (MIMAT0016915) miRNA expression lentivirusmiR#1164$800.00
hsa-miR-636 (MIMAT0003306) miRNA expression lentivirus miR#626$800.00
mmu-mir-200c-3p (MIMAT0000657) miRNA expression lentivirusmiR#3311$800.00

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