Non-Integrating Lentivirus express human codon-optimized wild-type Cas9 endonuclease (from Streptococcus Pyogenes, click to see its codon sequence) under the enhanced EF1a promoter, with our proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains a Puromycin antibiotic resistance that used for transient selection (within 1-2 weeks post-transduction) when desired. (See also the related regular lentivirus, CAT#: LVP684.
See Product Manual here (.pdf).
Application: transient delivery Cas9 protein for CRISPR editing, without the unwanted insertional mutagenesis
( Note: used with the “gene-specific gRNA lentivirus” that needed and not included, for CRISPR gene editing )
Amount: 5 x 106 IFU/ml x 200ul in PBS, pre-mixed with polybrene (10x, 60 ug/ml).
Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.