Transformed from human B lymphocyte cell line (RPMI 8226; Antigen Expression profile: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +), this cell line has the firefly Luciferase and RFP fluorescent report constitutively co-expressed under EF1a promoter as two separate proteins (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome.
Cells demonstrate strong RFP fluorescent signal, and luciferase assay signal via Luc assay (Note: It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven RFP signal intensity amount cells).
Sold at: 1 vial x (2 x 106 cells)/vial, see details in Product Manual.