Transformed from Mouse MB49 cell line, the firefly Luciferase 3 and RFP fluorescent report was constitutively co-expressed under the enhanced EF1a promoter as two separate proteins (not as fusion), mediated via F2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome.
Each cell demonstrates strong RFP fluorescent signal and luciferase/ chemiluminescence signal via Luc assay (Note: It is the pooled polyclonal population, not originated from a single cell colony. Thus you will observe the uneven RFP signal intensity amount cells).
Sold at: 1 vial x (2 x 106 cells)/vial, see details in Product Manual.