Premade shRNA lentivirus, knockdown human P53 gene (NM_000546). It contains a RFP-Puromycin dual selection marker. RFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Puromycin.
shRNA sequence was designed in human P53 codon (ORF) region, demonstrating greater than 75% knockdown levels for human P53 expression.
Note: Its matched negative control shRNA lentivirus is: CAT# H1(shRNA-Ctr)-RP.
Please see Product Manual for more details.
Amount: 200ul/tube at (1 x 10 7 IFU/ml) in DMEM medium with 10% FBS, 10 x polybrene (60 ug/ml),