West Nile virus is an enveloped a positive-stranded RNA virus in the family Flaviviridae. The virion consists of an envelope surrounding an icosahedral capsid of approximately 50 nm in size.
West Nile Virus (WNV) Envelope (click to see its protein sequence) Viral Like Particles was packaged in HEK293T cells using our proprietary technology. It is the replication-incompetent particles in which the WNV Envelope (with 6His-tag at its C-terminal) was pseudo-typed as the lentiviral particles’ surface envelope protein. [Note: This VLP has the WNV Envelope antigen expressed on the particle’s surface. If you want to over-express the WNV Envelope antigen in your desired cell types, please use lentivirus product, CAT#: LVP1410-PBS.]
This VLP packaged a non-sense sequence (Null) as the particle’s virion genomic material, to maintain the intact of the particles.
WNV Envelope was assembled as surface antigens which can be used as the ready to use WNV Envelope antigen, or for raising the specific antibodies, for the validation of anti-WNV Envelope antibodies, in vaccine development and other validation assays. This VLP can also be detected by the anti-C-terminal 6His-tag antibody.
The VLP’s titer was measured via ELISA P24 assay. Every single particle assembled many envelope molecules at its surface. See more details in Product Manual.
Amount: 200ul/vial at 1×108 VP/ml.