Puromycin is an antibiotic protein synthesis inhibitor which causes premature chain termination during translation. It derived from the Streptomyces alboniger bacterium.
Puromycin is used in cell biology as a selective agent in cell culture systems. It is toxic to prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the pac gene encoding a puromycin N-acetyl-transferase (PAC). Cells transduced with plasmids that containing puromycin resistant gene will survive in Puromycin selection, which is widely used in stable cell line generation.
The working concentration of Gentarget’s Puromycin solution were tested in many mammalian cell types against Gentarget’s lentivirus products containing puromycin resistance, see details in Product Manual (pdf)
Amount: 1.0 ml/tube at 5.0 mg/mL.