Blasticidin is a nucleoside antibiotic that is produced by Streptomyces griseochromogenes. It is used in cell biology as a selective agent to select transformed cells which have been engineered to carry a resistance gene for blasticidin.
It is toxic to prokaryotic and eukaryotic cells. Resistance to Blasticidin is conferred by BSD (the deaminase isolated from Aspergillus terreus) which is the most commonly used resistance gene to blasticidin. Cells transformed by plasmids that containing BSD gene will survive in Blasticidin selection, which is widely used in stable cell line generation.
The working concentration of Gentarget’s Blasticidin solution were tested in many mammalian cell types against Gentarget’s lentivirus products containing puromycin resistance, see details in Product Manual (pdf)
Amount: 1.0 ml/tube at 10.00 mg/mL.