Top quality, Best price and Fastest turnarund time, with >15 year lentivirus experience!
(1) shRNA lentiviral services:
We construct shRNA target knockdown lentivector by using either your provided shRNA or our designed shRNA sequences, for your genes of interest. We then generate 0.5 ml of each shRNA lentivirus, and ship you both the shRNA lentivector plasmid DNA and the ready-to-use shRNA lentivirus.
- You choose the shRNA lentivectors (see the vector map scheme below)
- We deliver the sequence verified shRNA lentivector and ready-to-use high-titer shRNA lentivirus, including the negative control shRNA (Neg-Control) .
Note: Knockdown validation is not included in this service, but if you order 3 shRNAs for one target, we guarantee either at least one shRNA showing >75% knockdown or a knockdown level of >75% by using the pooled three shRNA lentiviruses.
Price: $1400.00 USD for Four shRNAs (three target shRNA plus one negative control shRNA) lentiviruses (0.5ml/each) and vectors (5ug/each) with guaranteed knockdown.
Timeline: 1 ~ 2 weeks.
Citations: Huanhuan Joyce Chen (et al), J Clin Invest. 2012;122(9):3184-3196. Chemokine 25-induced signaling suppresses colon cancer invasion and metastasis.
(2) Over-Expression lentiviral services:
We construct the target expression lentivector with the features according to your request, and produce the ready-to-use over-expression lentivirus for your desired target.
- You choose the expression lentivector for the promoter and the marker (see the vector map scheme below) ;
- We sub-clone your target codon sequence into our lentivector;
- We deliver the high titer, ready-to-use lentivirus to you (note: the sub-cloned lentivector plasmid is not included);
You have the following options with this service:
- Promoters: suCMV, inducible CMV, EF1a, or CAG
- Selection markers: Blasticidin or Puromycin or antibiotic-fluorescent fusion dual marker
- Fluorescent tag-target fusion
- Any Tags at either the N- or C-terminus
- N- terminus fluorescent fusion marker (GFP, RFP or CFP);
- Or bicistronic expression where target and a fluorescent marker expressed under the same promoter as two separate proteins;
Note: Our optional inducible CMV promoter can be used for either constitutive or inducible expression.
Schematic representation of gene expression Lentiviral vectors
Citations: Stancy Joseph, et al. PLoS ONE 7(7): e42159 (2012). ARID3B Induces Tumor Necrosis Factor Alpha Mediated Apoptosis While a Novel ARID3B Splice Form Does Not Induce Cell Death.
Adnan Dibas, et al. IOVS, May 2012, Vol. 53, No. 6. Fluorescent Protein–Labeled Glucocorticoid Receptor alpha Isoform Trafﬁcking in Cultured Human Trabecular Meshwork Cells
(3) Signal reporter lentivirus services:
We construct the lentivector by sub-clone your desired promoter into our promoter-less lentivector, and deliver you the ready-to-use lentivirus.
- You choose the signal reporter type and selection marker(see the vector map scheme below) ;
- We sub-clone your promoter sequence into our lentivectors;
- We deliver the high titer, ready-to-use lentivirus to you ;
(4) miRNA expression and anti-miRNA lentivirus services:
We construct an anti-miRNA lentivector and produce ready-to-use lentivirus for inhibition of any miRNA listed in the miRBase database, or any your desired miRNA. You simply select the miRNA you want to inhibit, and we deliver the order within 7-10 business days.
click HERE for more details. See the miRNA expression lentivector scheme below.
(5) CRISPR / Cas9 lentivirus services:
Gentarget provide pre-made CAS9 expression lentivirus with different antibiotic / fluorescent markers, and design and construct gene specific guild RNA lentivectors (gRNA lentivectors), then produce ready-to-use targeting gRNA lentivirus for gene knockout, and optionally synthesize the single-strand or double-stranded loci specific repair insert or knock-in cassette for gene editing. See gRNA lentivector core map scheme below. You will receive (1) expression ready humanized CRISPR/Cas9 lentivirus, (2) a set of ready-to-use target gRNNA lentivirus, (3) when requested, the repair / knock-in / mutation / of DNA cassette or single strand repair DNA oligo. click HERE to see details about our CRISPR / CAS9 products.
Why use Gentarget’s Lentivirus Services:
- Take advantage of Gentarget’s extensive hand-on experiences in lentivirus system with many proprietary technologies;
- Ues the strongest prompter (suCMV) for the highest protein expression levels in mammalian cells;
- Select most advanced product features for Promoter, Fluorescent tag, Antibiotic selection for high virus titer;
- Fast turnaround time;
- Best quality and best price in the lentivirus fields;
How to order: