Skip to content

For product queries: Call 1-858-265-6446 or email at info@gentarget.com

| Login / Register | 0 items | $0.00

gentarget inc.

7930 Arjons Drive, Suite B
San Diego, CA 92126
United States

  • Home
  • Products
    • Premade Lentivirus
      • cDNA (ORF) Expression
      • Luciferases Lentivirus
      • Fluorescent Markers
      • Sub-cellular Imaging
      • Nano-Lantern Imaging
      • Cytoskeleton Imaging
      • Unstable GFP
      • near-infrared RFP
      • Fluorescent fusion
      • CRE recombinase
      • CRE, Flp ColorSwtich
      • SEAP Reporter
      • Tet Repressor
      • rtTA Expression
      • lacZ Enzyme
      • iPS stem cell factors
      • Other enzyme
    • Premade AAV Virus
    • Premade Adenovirus
      • Common Markers & Enzymes
      • GFP CFP YFP RFP BFP & Controls
      • iPS Stem Cell Factors
      • ORF Expression
    • Pathway Reporter
      • Androgen Pathway
      • Antioxidant Pathway
      • ATF6 Pathway
      • C/EBP Pathway
      • CD44CR1 Pathway
      • c-Myc Pathway
      • CREB (cAMP-PKA) Pathway
      • E2F Pathway
      • EGR1 Promoter Pathway
      • Estrogen Receptor Pathway
      • FOXO Pathway
      • GATA Pathway
      • Glucocorticoid Pathway
      • Hedgehog Pathway
      • HNF4 Pathway
      • Hypoxia Pathway
      • IRF Pathway
      • JAK-STAT Pathway
      • JNK / AP1 Pathway
      • MAPK/ERK Pathway
      • MEF2 Pathway
      • MTF1 Pathway
      • NFAT Pathway
      • Notch Signal Pathway
      • NFκB Pathway
      • PPAR Pathway
      • Progesterone Pathway
      • RAR Pathway
      • STAT3 Pathway
      • TGF-β Pathway
      • TP53 Pathway
      • Wnt Signal pathway
      • XBP1 Pathway
      • Blue light inducible reporter
      • Heat inducible expression
      • Mitochondrial pH-sensitive probe
      • Signal Pathway Control
    • Cell Immortalization
      • hTERT Expression
      • SV40 Large T antigen
      • Knockdown P53 shRNA Lentivirus
      • Other Immortalization Factors
    • CARs and TCRs
      • CAR-T Lentivirus
      • TCR Lentivirus
      • Cell Antigens & Receptors
    • CRISPR Gene Editing
      • Cas9 Expression
      • Epigenomic: CRISPRi and CRISPRa
      • gRNA Lentivirus
      • CRISPR Repair and KnockIn
      • CRISPR KnockOut
    • Stable cell lines
      • Assay Cell Lines
      • Luciferase cells
      • Fluorescent cells
      • CRE and Flp reporting cells
      • CRE Expression Cells
      • CRISPR ready cell lines
      • TetR stable cells
      • rtTA stable cells
      • Inducible expression cell lines
      • Ion Channel cells
      • LacZ stable cells
      • Other stable cells
    • Cell-Specific Reporter
      • Astrocytes Report
      • B-Cell Reporter
      • Brain Cell Report
      • Cancer Specific Report
      • Endothelial Cell Report
      • Hematopoietic Cell Report
      • Hepatoma Cell Report
      • Kidney Cell Report
      • Leukocytes Report
      • Lung Tissue Report
      • Macrophages Report
      • Megakaryocytes Report
      • Monocytes Report
      • Muscle Cell Report
      • Neuron Report
      • Ovarian Cancer Report
      • Pancreatic Cancer Report
      • Prostate Cell Report
      • Stem Cell Report
    • Infectious Antigens
      • Viral Antigens
    • Virus Like Particles (VLP)
    • Non-integrating LV
    • shRNA lentivirus
      • Validated shRNA Lentivirus
      • Customized shRNA
    • microRNA lentivirus
    • anti miRNA lentivirus
    • Lentivectors
      • CRISPR gRNA lentivectors
      • shRNA Lentivectors
      • Promoter-less Lentivectors
      • Lentivirus Packaging plasmids
    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
  • Services
    • Lentivirus Service
    • Customized Stable Cell Lines
    • Fusion In Vivo PCR Cloning
    • Protein Expression
    • Antibody Cloning and Expression
  • Support
    • Product citations
    • Technology
      • Optional Inducible Lentiviral System
      • SureTiter™ Lentiviral System
      • Enhanced super CMV promoter
      • InTag™ trans-membrane proteins
      • Fusion in vivo, Eco™ PCR Cloning
    • Protocols
    • MSDS Files
    • Promotion
    • Product Manuals
    • Vector sequences
    • Product FAQs
    • Terms
    • Useful Links
  • Distributors
  • About Us
  • Contact
  • News
Home / Premade Lentivirus / Tet Repressor

Tet Repressor

TetR (tetracycline regulator) for Inducible Expression:

TetR (tetracycline regulator) (click to see its codon sequence), is the key regulatory protein in the tetracycline-controlled inducible gene expression system.  When TetR protein is present it binds to the promoter, blocking expression. Induction of expression is accomplished by addition of tetracycline (or doxycycline) which removes TetR from the promoter.  In Tet-OFF system, it binds to promoter without Tetracycline (or Dox),  which activates the expression until Dox is absent which turn off the expression.

TetR Expression Lentivirus:

GenTarget provides optional inducible expression lentivirus in which the target or shRNA is driven by a modified CMV or H1 promoter. Those promoters have integrated with two copies of the TetR binding sequences. This modification does not change the promoter’s constitutive expression. Therefore, the target or shRNA can be expressed at a high level without induction, or by tetracycline induced expression, so called Optional inducible expression. 

So, when TetR present, you can turn those expression as inducible expression. GenTarget’s provides TetR expression lentivirus with a variety of antibiotic markers. TetR is expressed under either a super strong CMV promoter (producing the highest TetR protein level) or an enhanced EF1α promoter (a tissue nonspecific strong promoter with no silencing effects during long-term culture).

See the TetR expression vector map scheme below.

tet repressor expression scheme

Q: What is the difference between GenTarget’s inducible system and other inducible expression systems? 

A: The GenTarget system is an optional inducible system. 

GenTarget’s inducible lentivectors (shRNA vectors and expression vectors) have TetR binding sequences inserted into their constitutive promoter (H1 or CMV). This modification does not change the promoter’s constitutive expression property. The system becomes inducible only when the TetR protein is present to block expression. Addition of tetracycline removes TetR from the promoter, inducing expression.  

By comparison, other tetracycline-inducible expression systems use a silent promoter that has been modified to bind to a TetR.  Expression is turned on only when the TetR binds to the promoter  and turn off by Dox (Tet-OFF). The Tet-On and Tet-Off systems require a transcriptional activator (tTA or rtTA) along with tetracycline or Dox for inducible expression, but the Tet-on and Tet-off vector cannot be used alone for constitutive expression. 

Please see  Product’s Manual .

NameSKUPriceBuy
CMV-TetR (Bsd) Lentiviral particles in PBSLVP017-Bsd-PBS$450.00
CMV-TetR (GFP-Bsd) Lentiviral particles in PBSLVP017-GB-PBS$450.00
CMV-TetR (Hygro) Lentiviral particles in PBSLVP017-Hygro-PBS$450.00
CMV-TetR (Neo) Lentiviral particles in PBSLVP017-Neo-PBS$450.00
CMV-TetR (Puro) Lentiviral particles in PBSLVP017-Puro-PBS$450.00
CMV-TetR (RFP-Bsd) Lentiviral particles in PBSLVP017-RB-PBS$450.00
CMV-TetR (RFP-Puro) Lentiviral particles in PBSLVP017-RP-PBS$450.00
EF1a-TetR (Bsd) Lentiviral particles in PBSLVP459-Bsd-PBS$450.00
EF1a-TetR (GFP-Bsd) Lentiviral particles in PBSLVP459-GB-PBS$450.00
EF1a-TetR (Hygro) Lentiviral particles in PBSLVP459-Hygro-PBS$450.00
EF1a-TetR (Neo) Lentiviral particles in PBSLVP459-Neo-PBS$450.00
EF1a-TetR (Puro) Lentiviral particles in PBSLVP459-Puro-PBS$450.00
EF1a-TetR (RFP-Bsd) Lentiviral particles in PBSLVP459-RB-PBS$450.00
EF1a-TetR (RFP-Puro) Lentiviral particles in PBSLVP459-RP-PBS$450.00

    Cart

    Product Selection Guides

    • Luciferase Imaging
    • Cell Immortalization
    • Signal Pathway Assay
    • cDNA (ORF) Expression
    • CAR-T, TCR and More
    • CRISPR, CRISPRa, CRISPRi
    • Sub-cellular Imaging
    • Reporter Cell Lines
    • Premade Lentivirus
    • Promoter Research
    • Lentivirus & Cell Line Services

    VIEW MORE

    Stay Connected

    Online Payment Service
  • Home
  • Products
    • Premade Lentivirus
    • Premade AAV Virus
    • Premade Adenovirus
    • Pathway Reporter
    • Cell Immortalization
    • CARs and TCRs
    • CRISPR Gene Editing
    • Stable cell lines
    • Cell-Specific Reporter
    • Infectious Antigens
    • Virus Like Particles (VLP)
    • Non-integrating LV
    • shRNA lentivirus
    • microRNA lentivirus
    • anti miRNA lentivirus
    • Lentivectors
    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
  • Services
    • Lentivirus Service
    • Customized Stable Cell Lines
    • Fusion In Vivo PCR Cloning
    • Protein Expression
    • Antibody Cloning and Expression
  • Support
    • Product citations
    • Technology
    • Protocols
    • MSDS Files
    • Promotion
    • Product Manuals
    • Vector sequences
    • Product FAQs
    • Terms
    • Useful Links
  • Distributors
  • About Us
  • Contact
  • News

2008 ~ ©, 2025 GenTarget Inc. All rights reserved.

Contact | Terms | View Cart

Scroll Up