G418 sulfate (Geneticin) is an analog of neomycin sulfate, and has similar mechanism as neomycin. It is produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the neomycin gene from Tn5 encoding an aminoglycoside 3′-phosphotransferase, APT 3′ II.
It is used in cell biology as a selective agent to select transformed cells which have been engineered to carry a Neomycin resistance gene.
The working concentration of Gentarget’s Puromycin solution were tested in many mammalian cell types against Gentarget’s lentivirus products containing puromycin resistance, see details in Product Manual (pdf)
Amount: 1.0 ml/tube at 50 mg/mL.