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Home / Cell Immortalization

Cell Immortalization

Cell Immortalization Methods:

Normal cells will die after a few rounds of proliferation because of cellular senescence. There are a few methods to turn a primary cell to immortal so that the cells can undergo infinite cell divisions or large rounds of doubling in culture medium.

    1. One Cell Immortalization method is to repress the genes that control cell cycle such as such as retinoblastoma (Rb) and p53 genes (the tumor suppressor proteins). Many virus induced cancer cells have their viral genes repress “tumor suppressor proteins” and became immortal.  Over-expression of such viral genes can turn primary cells into immortal. The most common of these is the Large T-antigen of the simian virus (SV-40). The others of these are: Human Papilloma Virus (HPV)’s E6 and E7 genes, Epstein-Barr Virus (EBV), E1A gene of human adenovirus type 5, Ras_V12 mutant, cMyc and more. Also, siRNAs that knockdown p53 or Rb can also be used for this route.   
    2. Another Cell Immortalization method is to over-express the genes that confer immortality. The most well-known gene is Telomerase (hTERT) which is often found over-expressed in human tumors. Other those genes are HOX genes, CDK4 and more.

immortalization factor table

How to Select Cell Immortalization Reagents:

Depending on your cell types, you can combine both methods above to increase the immortalization efficiency or to immortalize a larger number of cells. Please be advised, after the immortalization, the phenotype of your primary cell may or may not be changed. And the immortalization is not necessarily oncogenesis. But, there are difference between primary cells and immortalized cells in terms of experimental model.

Lentivirus is the best and most efficiency delivery method for primary cell Immortalization. Gentarget provide most comprehensive cell immortalized lentivirus products, driven by different promoter and with different selection markers. see the lentivirus’ core structure showed in the scheme below:

cell immortalization lentivirus map

Please look into each product, or click the each product categories manuals links below:

  1. human TETR lentivirus;
  2. SV40 T-antigen lentivirus;
  3. Other genes for cell immortalization;
  4. P53 siRNA;
NameSKUPriceBuy
CDK4 (GFP-Puro) Expression LentivirusLVP1140-GP-PBS$650.00
CDK4 (RFP-Bsd) Lentivirus in PBSLVP1140-RB-PBS$650.00
cMyc (GFP-Puro) LentivirusLVP1141-GP$295.00
cMyc (GFP-Puro) Lentivirus in PBSLVP1141-GP-PBS$650.00
cMyc (RFP-Bsd) LentivirusLVP1141-RB$295.00
cMyc (RFP-Bsd) Lentivirus in PBSLVP1141-RB-PBS$650.00
E1A (GFP-Puro) Lentivirus in PBSLVP1137-GP-PBS$650.00
E1A (RFP-Bsd) Lentivirus in PBSLVP1137-RB-PBS$650.00
EBNA1 (GFP-Puro) Lentivirus in PBSLVP1134-GP-PBS$650.00
EBNA1 (RFP-Bsd) Lentivirus in PBSLVP1134-RB-PBS$650.00
EBNA2 (GFP-Puro) Lentivirus in PBSLVP1135-GP-PBS$650.00
EBNA2 (RFP-Bsd) Lentivirus in PBSLVP1135-RB-PBS$650.00
h KRas_G12V (GFP-Puro) Lentivirus in PBSLVP1139-GP-PBS$650.00
h KRas_G12V (RFP-Bsd) Lentivirus in PBSLVP1139-RB-PBS$650.00
HOXA9 (GFP-Puro) Lentivirus in PBSLVP1138-GP-PBS$650.00
HOXA9 (RFP-Bsd) Lentivirus in PBSLVP1138-RB-PBS$650.00
HpV16-E6 (GFP-Puro) Lentivirus in PBSLVP1136-GP-PBS$650.00
HpV16-E6 (RFP-Bsd) Lentivirus in PBSLVP1136-RB-PBS$650.00
hTERT (CMV Promoter), Concentrated LentivirusLVP1130-PBS$650.00
hTERT (CMV, Bsd) lentivirus in PBSLVP1130-Bsd-PBS$650.00
hTERT (CMV, GFP-Bsd) lentivirus in PBSLVP1130-GB-PBS$650.00
hTERT (CMV, GFP-Puro) lentivirusLVP1130-GP$350.00
Read more
hTERT (CMV, GP), Concentrated lentivirusLVP1130-GP-PBS$650.00
hTERT (CMV, Hygro) lentivirus in PBSLVP1130-Hygro-PBS$650.00
hTERT (CMV, Neo) lentivirus in PBSLVP1130-Neo-PBS$650.00
hTERT (CMV, Puro) lentivirus in PBSLVP1130-Puro-PBS$650.00
hTERT (CMV, RFP-Bsd) lentivirus in PBSLVP1130-RB-PBS$650.00
hTERT (CMV, RFP-Puro) lentivirusLVP1130-RP$350.00
Read more
hTERT (CMV, RFP-Puro) lentivirus in PBSLVP1130-RP-PBS$650.00
hTERT (CMV, Zeo) lentivirus in PBSLVP1130-Zeo-PBS$650.00
hTERT (EF1a Promoter), Concentrated lentivirusLVP1131-PBS$650.00
hTERT (EF1a, Bsd) lentivirus in PBSLVP1131-Bsd-PBS$650.00
hTERT (EF1a, GFP-Bsd) lentivirus in PBSLVP1131-GB-PBS$650.00
hTERT (EF1a, GP) lentivirus in PBSLVP1131-GP-PBS$650.00
hTERT (EF1a, Hygro) lentivirus in PBSLVP1131-Hygro-PBS$650.00
hTERT (EF1a, Neo) lentivirus in PBSLVP1131-Neo-PBS$650.00
hTERT (EF1a, Puro) lentivirus in PBSLVP1131-Puro-PBS$650.00
hTERT (EF1a, RFP-Bsd) lentivirus in PBSLVP1131-RB-PBS$650.00
hTERT (EF1a, RFP-Puro) lentivirus in PBSLVP1131-RP-PBS$650.00
hTERT (EF1a, Zeo) lentivirus in PBSLVP1131-Zeo-PBS$650.00
Knockdown Lentivirus, shRNA (h P53) / (RFP-Bsd)LVP343-RB-PBS$650.00
Knockdown Lentivirus, shRNA (h P53) / (RFP-Puro)LVP343-RP-PBS$650.00
Knockdown Lentivirus, shRNA (h P53) /(GFP-Bsd)LVP343-GB-PBS$650.00
Knockdown Lentivirus, shRNA (h P53) /(GFP-Puro)LVP343-GP-PBS$650.00
SV40 large T-antigen (CMV, Bsd) lentivirus in PBSLVP016-Bsd-PBS$650.00
SV40 large T-antigen (CMV, GFP-Bsd) lentivirus in PBSLVP016-GB-PBS$650.00
SV40 large T-antigen (CMV, GFP-Puro) lentivirus in PBSLVP016-GP-PBS$650.00
SV40 large T-antigen (CMV, Hygro) lentivirus in PBSLVP016-Hygro-PBS$650.00
SV40 large T-antigen (CMV, Neo) lentivirus in PBSLVP016-Neo-PBS$650.00
SV40 large T-antigen (CMV, No Selection) lentivirus in PBSLVP016-PBS$650.00
SV40 large T-antigen (CMV, Puro) lentivirus in PBSLVP016-Puro-PBS$650.00
SV40 large T-antigen (CMV, RFP-Bsd) lentivirus in PBSLVP016-RB-PBS$650.00
SV40 large T-antigen (CMV, RFP-Puro) lentivirus in PBSLVP016-RP-PBS$650.00
SV40 large T-antigen (CMV, Zeo) lentivirus in PBSLVP016-Zeo-PBS$650.00
SV40 large T-antigen (EF1a, Bsd) lentivirus in PBSLVP557-Bsd-PBS$650.00
SV40 large T-antigen (EF1a, GFP-Bsd) lentivirus in PBSLVP557-GB-PBS$650.00
SV40 large T-antigen (EF1a, GFP-Puro) lentivirus in PBSLVP557-GP-PBS$650.00
SV40 large T-antigen (EF1a, Hygro) lentivirus in PBSLVP557-hygro-PBS$650.00
SV40 large T-antigen (EF1a, Neo) lentivirus in PBSLVP557-Neo-PBS$650.00
SV40 large T-antigen (EF1a, No Selection) lentivirus in PBSLVP557-PBS$650.00
SV40 large T-antigen (EF1a, Puro) lentivirus in PBSLVP557-Puro-PBS$650.00
SV40 large T-antigen (EF1a, RFP-Bsd) lentivirus in PBSLVP557-RB-PBS$650.00
SV40 large T-antigen (EF1a, RFP-puro) lentivirus in PBSLVP557-RP-PBS$650.00
SV40 large T-antigen (EF1a, Zeo) lentivirus in PBSLVP557-Zeo-PBS$650.00

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