Fluorescent fusion

Lentivirus Expression of “Fluorescent-Target” Fusion Proteins

Simplify expression of “fluorescent-Target” fusion proteins using GenTarget’s Pre-Made Lentiviral Particles.These particles allow easy high-titer expression of a target protein fused to Green, Blue, Red, or Cyan Fluorescent Protein.  GenTarget’s Premade Lentiviral Particles exhibit brighter fluorescence and stronger transduction efficiency than competing products, and are ideal for use in difficult-to-transduce cells. They allow quick selection by either antibiotic resistance or fluorescence, and may be used singly or in multi-color applications.  

Pre-made Lentiviral Particles are generated using GenTarget’s High Expression Lentiviral System in which a target gene is in frame with a fluorescent marker, GFP, RFP, or CFP expressed as a covalent bond fusion protein.  Expression is driven from a proprietary suCMV promoter that demonstrates the highest expression levels (3-10 fold higher than the CMV promoter in the pCDNA6.3 vector, depending upon cell type) and each fluorescent tag is codon optimized to generate the brightest signal. An antibiotic selection marker (Puromycin, Blasticidin or Neomycin) is expressed under the RSV promoter. See map scheme below. 

fusion-vector-map

Gentarget’s premade lentiviral particles are best in the class, demonstrating the brightest fluorescent signal, strong transduction efficiency. Each particles was validated in lot by lot basis and its quality is guaranteed. Particles are provided in DMEM medium with 10% FBS and 60ug/ml polybrene, or concentrated and resuspended into PBS solution, as ready to use status. Note: higher titer viruses are available upon special requests.

Key features:

  • Robust Expression and High Titer: GenTarget’s Premade Lentiviral Particles have the brightest fluorescence and the strongest transduction efficiency of any lentiviral particles on the market.
  • Long-Term Expression: GenTarget’s Premade Lentiviral Particles produce long-lasting expression of fluorescently-labeled target proteins even in hard-to-transfect cell lines such as primary and neuronal cells.
  • Easy Transduction: Simply add the Particles to your cell culture and visualize fluorescence in 48-92 hours. There is no need for any additives or changes of medium.
  • Multiple Colors: Particles expressing different colors may be transduced into the same cells for multi-color applications.
  • Easy Selection of Transduced Cells:  Use either fluorescence signal or puromycin / Blasticidin / Neomycin resistance.
  • Tested and Validated: Each lot of Particles is validated and guaranteed to be of the highest quality.
  • Ready-to-Use:  Particles are provided in DMEM medium with 10 % FBS and 60 µg/ml polybrene (10x). 

 For general questions about our ready-to-use lentiviral particles, please See FAQ for pre-made lentiviral particles (.pdf)

If you want to label your specific target, Gentarget Inc also provides lentiviral services for making the fusion protein of your gene of interest and a fluorescent marker, and generates ready-to-use viral particles with the best prices and fastest turnaround time. 

Please click links below for each product’s details, or click here for product manual.

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