Lentivirus express a fusion target of (GFP-Annexin V). The full length coding sequence of human Annexin V (NCBI accession ID: NM_001154 ) was fusioned with GFP (Green Fluorescent Protein), at its N-terminal, expressed under an enhanced suCMV promoter, containing a Puromycin antibiotic marker under the RSV promoter.
Fluorescent labelled Annexin V (or Annexin 5) can be used to detect early apoptotic cells. Annexin 5 interacts strongly and specifically with phosphatidylserine (PS) that exposed from plasma membrane in the early event in apoptosis. Therefore, Annexin 5 can be used to detect apoptosis by targeting for the loss of plasma membrane asymmetry. Labeled annexin V can be applied both in flow cytometry and in light microscopy in living culture culture.
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Amount: 200ul/vial (1×108 IFU/ml), provided in DMEM medium, containing 60ug/ml of Polybrene (10x).