Pre-made lentiviral particles expressing a fusion target of (GFP-Annexin5). The sequence verified full lengt human Annexin5-ORF (NCBI accesion ID: NM_001154 ) was fusioned in frame with GFP at its N-terminal, expressed under a consitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter.
Fluorescent labelled Annexin 5 (or Annexin V) can be used to detect early apoptotic cells. Annexin 5 interacts strongly and specifically with phosphatidylserine (PS) that exposured from plasma membrane in the early event in apoptosis. Therefore, Annexin 5 can be used to detect apoptosis by targeting for the loss of plasma membrane asymmetry. Labeled annexin V can be applied both in flow cytometry and in light microscopy in living culture culture.
Please see product manual for details.
Amount: 200ul/vial (1×108 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).